FIG. 6.

Expression of activated AKT; MEK or Bcl-2 protects IL-3-deprived 32D cells from elevation of mitochondrial ROS and Ca²⁺. (A) Cells were deprived of IL-3 through extensive washing and 15 to 16 h later were labeled with MitoSOX Red or Rhod-2. * and **, significantly different from corresponding vector values (P < 0.05 and P < 0.01, respectively; n = 3). (B). 32D Bcl-2, AKT, MEK, and vRAF cells and the corresponding vector cells (G [GAPDH]) were washed two times with PBS and directly lysed in Laemmli buffer and processed for immunoblotting as described in Materials and Methods.