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Effect of oncogenic RAF on the expression of Bcl-2 and antioxidant proteins. 32D and 32D-vRAF cells were incubated with or without IL-3 for 8 h and then washed two times with PBS, directly lysed in Laemmli buffer, and processed for immunoblotting as described in Materials and Methods. Lanes: 1, 32D minus IL-3; 2, 32D plus IL-3; 3, 32D-vRAF minus IL-3; 4, 32D-vRAF plus IL-3.
