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. 2008 Jan 28;28(7):2113–2124. doi: 10.1128/MCB.02136-07

FIG. 2.

FIG. 2.

Nap1 is not essential for Yra1 nuclear import and Yra1-mediated mRNA export. (A) pYra1-GFP2 or pH2A1-46-GFP2 was expressed in wild-type or Δnap1 cells cotransformed with pRS425GPD or pRS425GPD-NAP1 and visualized by fluorescence microscopy. The coincident Hoechst images are shown. (B) Wild-type or Δnap1 cells were transformed with pRS425GPD or pRS425GPD-NAP1. mRNA localization was determined by poly(A) FISH using cells fixed at 30°C. The coincident Hoechst images are shown. (C) mRNA was localized by poly(A) FISH as described above, using strains of the indicated genotypes, after incubation at the indicated temperatures.