FIG. 5.

TAZ induces the EMT of MCF10A cells. (A) TAZ induces a morphology change in MCF10A cells. Phase-contrast images of MCF10A cells expressing vector, TAZ, TAZ^S89A, and TAZ^4SA are shown. WT, wild type. (B) TAZ alters the actin organization. Cells were stained with rhodamine-conjugated phalloidin. (C) TAZ causes EMT in MCF10A cells. Cell lysates from MCF10A cells expressing vector, TAZ, and TAZ^4SA were separated and probed with antibodies for epithelial markers and mesenchymal markers as indicated. (D) TAZ decreases E-cadherin and increases N-cadherin expression through the activation of EMT transcription. Total RNA was extracted from MCF10A-vector, -TAZ, and -TAZ^4SA cells, and quantitative PCR was performed to determine E-cadherin and N-cadherin expression (upper panel) and Foxc2 and Snail expression (lower panel). All data are normalized to GAPDH.