FIG. 4.

Ectopic expression of degradation-resistant Rev-erbα blocks adipocyte differentiation. (A) Preadipocytes expressing WT or SD Rev-erbα expression vectors were differentiated for 9 days with or without 2 μg/ml doxycycline (Dox) and stained with Oil Red O. (B) aP2 mRNA levels in cells expressing ectopic WT and SD Rev-erbα. (C) aP2 protein levels in cells expressing ectopic WT and SD Rev-erbα. β-Actin served as a loading control. (D) Expression of the ectopic SD and WT Rev-erbα proteins during adipogenesis. β-Actin served as a loading control.