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Rev-erbα expression represses PPARγ2. (A) Ectopic expression of degradation-resistant (SD), but not WT, Rev-erbα blocks PPARγ2 induction. (B) Retroviral expression of ectopic PPARγ2 in 3T3-L1 preadipocytes. (C) Ectopic expression of PPARγ2 rescues adipogenesis in 3T3-L1 cells ectopically expressing degradation-resistant SD Rev-erbα, as assessed by Oil Red O staining on day 7. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
