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. 2008 Jan 22;28(7):2125–2137. doi: 10.1128/MCB.00740-07

FIG. 1.

FIG. 1.

The effect of EWS/ETS on the morphology of UET-13 cells. (A) The establishment of a tetracycline-inducible EWS/ETS expression system in UET-13 cells. CMV, cytomegalovirus. (B) Analyses for confirming the inducible expression of EWS/ETS genes. EWS/ETS mRNAs were detected in UET-13 transfectants UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG by RT-PCR. These cells were treated with or without 3 μg/ml of tetracycline (Tet) for the indicated periods. As an internal control, a human GAPDH gene was used. (C) Analyses for confirming the inducible expression of EWS/ETS proteins. The cells were treated as described for panel B and subjected to Western blotting for the detection of EWS/ETS proteins. The extracts of RD-ES and W-ES cells were also examined as positive controls. Membranes were reprobed with anti-actin antibody as a loading control. (D) Morphological change after tetracycline treatment of UET-13 transfectants. UET-13 cells and the transfectants were cultured in the absence or presence of tetracycline for 72 h and observed by light microscopy. Magnification, ×40 (top); ×200 (bottom). Cells were also examined using hematoxylin-eosin (HE) (top) and May-Giemsa (bottom) staining (magnification, ×200).