FIG. 2.
Effects of EWS/ETS on cell growth in UET-13 cells. (A) Growth curve for UET-13 transfectants. Cells were seeded at 103/well and cultured as described for Fig. 1. The increase in cell number was analyzed by MTT assay. Values are means with the standard errors (SE) from three independent experiments. Diamond symbols indicate UET-13 transfectants in the absence of tetracycline (Tet); box symbols indicate UET-13 transfectants in the presence of tetracycline. (B) Cells were cultured as described for panel A in the absence or presence of tetracycline for 3 days and then stained with PI, and DNA contents were analyzed by flow cytometry (x axis, relative intensity of fluorescence; y axis, relative cell number). (C) Cells treated as described for panel B were stained with FITC-annexin V and analyzed.