FIG. 5.

Immunophenotypic change on induction of EWS/ETS expression in UET-13 cells. UET-13 transfectants were cultured with or without 3 μg/ml of tetracycline for 1 week and flow cytometric analyses were performed by using a set of antibodies as indicated. The histograms of UET-13 transfectants with (empty) and without (gray) tetracycline treatment were overlaid. Dotted lines indicate fluorescence intensities in negative control panels (Cnt). Arrows indicate the immunophenotypic change caused by tetracycline. The immunophenotypes of the EFT cell lines RD-ES and SK-ES1 were also examined.