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. 2008 Jan 22;28(7):2125–2137. doi: 10.1128/MCB.00740-07

FIG. 6.

FIG. 6.

The change of expression profile on induction of EWS/ETS in UET-13 cells. UET-13TR-EWS/FLI1 and UET-13TR-EWS/ERG cells were cultured in the absence or presence of tetracycline (Tet) for the indicated periods and analyzed using the Affymetrix human genome U133 Plus 2.0 array as described in Materials and Methods. (A) The sequential changes of ID2, NKX2.2, and IGFBP3 mRNA levels in UET-13 transfectants upon treatment with or without tetracycline. Diamond symbols indicate UET-13 transfectants in the absence of tetracycline; box symbols indicate UET-13 transfectants in the presence of tetracycline. (B and C) Microarray studies for the determination of expression profiles of surface antigens in UET-13 transfectants. UET-13 transfectants were treated with or without 3 μg/ml of tetracycline for 72 h. mRNA levels were determined with the Affymetrix human genome U133 Plus 2.0 array.