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Effects of EWS/ETS expression on the Matrigel invasion ability of UET-13 cells. UET-13TR (A), UET-13TR-EWS/FLI1 (B), and UET-13TR-EWS/ERG (C) cells were cultured in the absence or presence of tetracycline (Tet) for 72 h and then plated (2.5 × 104) on Matrigel-coated or uncoated filter inserts. After 20 h of culture, invading cells were stained with hematoxylin-eosin and counted in five fields per membrane as described in Materials and Methods. *, P < 0.05.
