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pUL117 was required for efficient accumulation of representative late viral proteins during HCMV infection. HF were infected with ADwt or ADinUL117C19-1nt at a multiplicity of infection of 0.1 PFU/cell; cells were harvested at different times postinfection; and accumulations of immediate-early proteins (IE1 and IE2), early protein (pUL44), and late proteins (MCP and pp28) were determined by Western blotting analysis. The antibody to actin was used as a loading control.
