FIG. 2.

Deletion of the UL117 gene locus resulted in severely attenuated growth of HCMV. HF were infected at a multiplicity of infection (moi) of either 0.01 PFU/cell (for multistep growth analysis) or at 2 PFU/cell (for single-step growth analysis) with various recombinant HCMV viruses as indicated. Culture medium was collected on different days postinfection, and yields of cell-free virus were determined by plaque assay. ADrevUL117-1 and ADrevUL117-2 are marker-rescued viruses of ADdlUL117 and ADinUL117, respectively.