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. 2008 Jan 23;82(7):3329–3341. doi: 10.1128/JVI.02104-07

FIG. 3.

FIG. 3.

Analysis of relative cell surface levels of GP64 chimeras. (A) Relative cell surface levels of chimeric GP64 proteins containing TM domain substitutions were measured by cELISA, using MAb AcV5. Values represent the means from triplicate transfections and are normalized to that for 7.5 μg of plasmid DNA expressing wt GP64. Error bars represent the standard deviations from the means. The amount of DNA used per transfection is indicated below the graph. (B and C) Immunofluorescence analysis of cell surface levels of GP64 mutants, using MAb AcV5 (B) or AcV1 (C). Cell surface GP64 was detected in transfected Sf9 cells fixed with 4% paraformaldehyde (B [left] and C), and total cell GP64 was detected in Sf9 cells fixed with methanol (B [right]). Cells in panel C were transfected with plasmids expressing the following constructs: a, mock transfected; b, wt GP64; c, GP64TM-CD4; d, GP64TM-GpA; e, GP64TM-HA; f, GP64TM-OpF; g, GP64TM-OpGP64; and h, GP64TM-GP75.