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. 2008 Jan 30;82(7):3561–3573. doi: 10.1128/JVI.02080-07

FIG. 6.

FIG. 6.

Epitope mapping of p24 in nonstimulated and DC h-HxB2 gag-stimulated PBL. Freshly thawed PBL (black bars) or PBL cocultured for 1 week with DC h-HxB2 gag (white bars) were stimulated with single peptides representing p24 (34 to 76) or with the pool of peptides encompassing whole p24 (p24) or whole Gag (Gag). The first peptide is number 34 (PIVQNLQGQMVHQAI), and the last one is 76 (YKTLRAEQASQEVKN). A positive response was defined as a minimum of 50 SFC/million responder cells, a ratio of a minimum of 4 between the responses of T cells stimulated with DC mock and with DC h-HxB2 gag, and in addition, more than three times the standard deviation above the mock-stimulated mean. This experiment was done for six HAART donors (P59 [A], P60 [B], P61 [C], P62 [D], P63 [E], and P64 [F]).