FIG. 7.

HIV-specific T cells can be expanded by autologous proviral gag mRNA-electroporated DC. MO-DC from nine HIV-1-seropositive subjects under HAART (P49 to 57) were electroporated with mRNA encoding wt-HxB2 (DC HxB2 gag) or autologous proviral gag (DC aut gag). These DC were matured and cocultured for 1 week with autologous PBL. Afterward, these PBL were stimulated with the HxB2 Gag peptide pool (day 7), and their responses were compared with those of freshly thawed PBL directly stimulated with the HxB-2 Gag peptide pool (day 0). The results are shown as individual data points representing the proportion of PBL from that person that secrete IFN-γ (A), IL-2 (B), or perforin (C). The P values of statistically significant differences between groups are as indicated in the legend for Fig. 1.