FIG. 3.

Entry of HCVpp into endothelial cells expressing various human claudins. 293T cells stably expressing claudin-1 (A), claudin-6 (B), or claudin-9 (C) or Huh-7.5 cells (D) were infected with HCVpp comprising E1E2 envelope glycoproteins derived from the HCV subtypes indicated along the x axis. Luciferase activity (in RLU) in cell lysates was measured 48 h postinfection. Background levels of entry of various HCVpp into 293T cells transduced by a control vector were 100 to 500 RLU and were subtracted from RLU values obtained for the claudin-positive cell lines for every experiment. Values are the means ± standard deviations of the results of three independent experiments performed with different viral stocks.