FIG. 6.

Claudin-6 and claudin-9 are weak HCVpp entry cofactors in Huh-7.5 cells. Huh-7.5 cell derivatives expressing a neomycin resistance marker (XIN), claudin-6, or claudin-9 were transfected by siRNAs targeting claudin-1 and infected with HCVcc-Rluc, HCVpp, or VSVpp. Luciferase activity (in RLU) was measured in cell lysates 48 h postinfection, and percent viral entry was calculated relative to the RLU obtained with the control siRNAs. Values are the averages ± standard deviations of the results of four independent experiments. *, statistically significant increase in virus or pseudoparticle entry compared to entry into XIN control cells; P < 0.02 as calculated by the two-tailed paired t test. α, anti.