FIG. 3.

Characterization of immature and mature human DCs. Monocytes were treated for 6 days with IL-4 and granulocyte-macrophage colony-stimulating factor and then either rested in the same medium for 1 more day (immature DC) or treated with activation cocktail (IL-1β, IL-6, tumor necrosis factor alpha, and PGE2) for 1 more day (mature DC). (A) Light microscopic views (×100 magnification) of two monocyte-derived DCs are shown. (B) Granularity and surface expression results for the indicated markers (HLA-DR, Lin1, and CD80) in immature and mature DCs are also shown.