FIG. 1.

3D reconstruction of CAV-2 at 12-Å resolution and comparison with the previously determined structure of hAdV-5 (MSD accession number 1111) at 10-Å resolution. (A) Isosurface representation of CAV-2 as viewed down the threefold axis. The pentons are shown in petrol blue, and the rest of the capsid is shown in yellow. The icosahedral axes are indicated by an ellipse (twofold), a triangle (threefold), and a pentagon (fivefold). A continuous line connecting the three pentons pointing in your direction delimits a facet. (B) A central slice through the CAV-2 EM reconstruction showing that the capsid density is well defined. One of the fibers sticking out radially from the capsid is indicated by a star. A circle surrounds the nonattributed extra density present under the penton base. The arrow indicates protein IX density. Scale bar, 10 nm. (C) Isosurface representation of hAdV-5 view down the threefold axis. The pentons are shown in blue, and the rest of the capsid is shown in gray. (D) A central slice through the hAdV-5 EM reconstruction showing that the capsid density is well defined. The fibers sticking out radially from the capsid are indicated by a star. The RGD loop is indicated by an arrow. Scale bar, 10 nm.