FIG. 5.

The minor protein IX. (A) Detailed view of one facet of CAV-2 seen down the threefold axis of the virion. The pentons are located on the vertices of the triangle and are shown in petrol blue, and the hexons are shown in pale yellow as in Fig. 1. Additional densities present as four groups of trimeric triskelions assigned to be protein IX are yellow (N-terminal part) and red (C-terminal part). (B) The top part represents the center of the facet described as in panel A but slightly tilted to see the extension of the C terminal part of protein IX form its N terminal part. The lower part is a slice through the capsid along the line shown in panel A. The color codes are the same as in panel A. (C) Detailed view of one facet of hAdV-5 seen down the threefold axis of the virion. The pentons are located on the vertices of the triangle and are in blue, and the hexons are gray. Protein IX is shown in yellow, and the cylindrical density that we initially assigned to protein IIIa (15) but that was recently reassigned to the C-terminal part of protein IX (45) is in red. (D) Detailed view of the threefold axis of the CAVpIX-GFP reconstruction. The penton and the hexons are as described in panel A. Protein IX is in yellow (N-terminal part) and red (C-terminal part), and the extra density assigned to GFP is in green. (E) Sequence alignment of hAdV-5 and CAV-2 protein IX. Conserved residues are in white text and highlighted in red; partially conserved residues are in red text. The arrows and open bars represent predicted β-strands and α-helices, respectively, as given by the secondary structure prediction software (http://bioweb.pasteur.fr/seqanal/interfaces/predator-simple.html). The predicted coil-coiled is boxed into a rectangle.