FIG. 3.

Kinetics of virus-specific CD8⁺ T-cell responses in tissues of Cbl-b^−/− and B6 mice with acute or chronic infection. Cbl-b^−/− or B6 mice were infected with 10² (A) or 2 × 10⁶ (B) PFU of LCMV Docile, and total numbers of virus-specific CD8⁺ T cells (sums of GP1_33-41 and NP_396-404 peptide-specific T cells) were determined by staining with D^b/GP1_33-41 and D^b/NP_396-404 tetramers (•). The total numbers of virus-specific CD8⁺ T cells from tissues were tested for their ability to produce IFN-γ following short-term culture with virus-infected DC2.4 cells on the indicated days after infection (○). Data shown are mean ± SEM log₁₀ virus-specific T cells per tissue for 5 to 10 mice.