FIG. 4.

Tissue-specific kinetics of virus-specific CD8⁺ T-cell responses expressed as percentages of CD8⁺ T cells (tetramer or IFN-γ positive) in Cbl-b^−/− and B6 mice with acute or chronic infection. The percentages of total CD8⁺ T cells specific for GP1_33-41 and NP_396-404 determined by tetramer staining in indicated tissues were calculated for acute (10² PFU) (panel A) or chronic (2 × 10⁶ PFU) (panel B) infection with LCMV Docile of Cbl-b^−/− (▴) or B6 (•) mice. In addition, the percentages of CD8⁺ T cells producing IFN-γ following short-term culture with virus-infected DC2.4 cells are indicated. Data shown are mean ± SEM log₁₀ virus-specific T cells per tissue for 5 to 10 mice.