FIG. 6.

Bcl-2 or Bcl-xL expression in virus-specific CD8⁺ T-cell populations generated in Cbl-b^−/− or B6 mice with acute or chronic infection. Spleen cells from Cbl-b^−/− (thin lines) or B6 (bold lines) mice infected with 10² or 2 × 10⁶ PFU of LCMV Docile 9 or 16 days prior were stained for intracellular Bcl-2 or Bcl-xL by using directly conjugated PE or allophycocyanin antibody after surface staining with anti-CD8α and D^b/GP1_33-41 or D^b/NP_396-404 tetramers. Spleen cells from naive Cbl-b^−/− or B6 mice were stained for CD8, CD44, and intracellular Bcl-2 or Bcl-xL. The histograms are gated on CD8 and D^b/GP1_33-41 or D^b/NP_396-404 tetramer-binding T cells (infected) or CD8⁺-CD44^low (naïve), and the numbers are the mean fluorescence intensities for Bcl-2 or Bcl-xL staining of B6 or Cbl-b^−/− mice. Data are for two mice per group and are representative of two experiments.