FIG. 9.

Immune-mediated pathology in Cbl-b^−/− mice infected with a moderate dose of LCMV Docile. (A) Cbl-b^−/− or B6 mice were infected with 10⁴ PFU of LCMV Docile, and spleen, lung, liver, and kidney tissues recovered on day 7 p.i. were fixed in acetone, sectioned, and stained with hematoxylin and eosin. For spleens and livers, low- and high-magnification images are presented. Arrows in the micrographs indicate intact and destroyed lymphoid follicles in B6 and Cbl-b^−/− infected mice (for spleens), inflammatory reaction in Cbl-b^−/− mice associated with extensive destruction of cells centered on portal tract in a hepatic lobus (for livers), and glomeruli with scattered mononuclear cell infiltrates in both infected Cbl-b^−/− and B6 mice (for kidneys). (B) Liver-specific enzyme activity (sALT and sAST) in Cbl-b^−/− (filled bars) or B6 (open bars) mouse sera collected on days 5 and 7 p.i. with 10⁴ PFU of Docile was measured. Control serum values from uninfected mice are indicated (control). Data shown are mean ± SEM enzymatic activity levels (U/liter) for three to six individual mice.