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. 2008 Jan 30;82(7):3679–3688. doi: 10.1128/JVI.02301-07

FIG. 4.

FIG. 4.

Zta-mediated induction of IL-8 is independent of Egr-1. (A) NPC-TW01 cells transfected with the vector plasmid (V) or a Zta-expressing plasmid (Z) were cotransfected with no siRNA (mock), Egr-1-targeted siRNA (siEgr-1), or a control siRNA (siCtrl). The culture supernatants were subjected to quantification of IL-8 by using ELISA, and the cell lysates were analyzed for the expression of Egr-1, Zta, and β-actin in an immunoblotting assay. (B) HONE-tetonZ cells were transfected with no siRNA (mock), Egr-1-targeted siRNA (siEgr-1) or a control siRNA (siCtrl) and treated with (+) or without (−) doxycycline (dox). The culture supernatants and cell lysates were subjected to the same experiments described in panel A.