TABLE 1.
Function and primer | Nucleotide sequence (5′→3′) | Position | Direction |
---|---|---|---|
PCR | |||
P1 | TTTTTCACCTCTGCCTAATCA | 1821-1841 | Sense |
P2 | CCCTAGAAAATTGAGAGAAGTC | 262-283 | Antisense |
P3a | CCACTGCATGGCCTGAGGATG | 3193-3213 | Antisense |
P4 | GCCTCATTTTGTGGGTCACCATA | 2801-2824 | Sense |
P5 | TTCTTTGACATACTTTCCA | 979-997 | Antisense |
P6a | TTGGGGTGGAGCCCTCAGGCT | 3070-3090 | Sense |
P7a | TTGGCCAAAATTCGCAGTC | 300-318 | Sense |
P8a | CCCCACTGTTTGGCTTTCAG | 714-734 | Sense |
P9a | GTTGATAAGATAGGGGCATTTGGTGG | 2299-2325 | Antisense |
Sequencing | |||
S1 | CTCCGGAACATTGTTCACCT | 2031-2050 | Sense |
S2 | AAGGTGGGAAACTTTACTGGGC | 2469-2490 | Sense |
S3 | GCTGACGCAACCCCCACTGG | 1186-1205 | Sense |
S4 | TCGCATGGAGACCACCGTGA | 1604-1623 | Sense |
S5 | GGCAAAAACGAGAGTAACTC | 1940-1959 | Antisense |
S6 | GGGTCGTCCGCGGGATTCAG | 1441-1460 | Antisense |
S7 | GACATACTTTCCAATCAATAGG | 970-991 | Antisense |
S8 | GAAGATGAGGCATAGCAGCAGG | 411-433 | Antisense |
S9 | CATGCTGTAGCTCTTGTTCC | 2831-2850 | Antisense |
Primer also used for sequencing.