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. 2008 Jan 16;82(7):3654–3664. doi: 10.1128/JVI.01888-07

FIG. 4.

FIG. 4.

The CTAR1 domain of LMP1 is responsible for ERK-MAPK and Akt activation. (A) Immunoblot analyses of MDCK control cells and cells expressing wild-type and mutant LMP1 proteins for LMP1 (upper panel), p-ERK1/2 (upper middle panel), total ERK1/2 (middle panel), p-Akt (lower middle panel), and Akt (bottom panel). Control cells stimulated with 100 ng of EGF/ml for 5 min were included as positive controls. The increases (n-fold) in ERK-MAPK and Akt phosphorylation are indicated in parentheses. p-p44, phosphorylated p44; p-p42, phosphorylated p42. (B) Control and LMP1-expressing cells were cultured in medium containing 0.5% FCS and either a vehicle control (DMSO) (upper panel), 20 μM U0126 (upper middle panel), or 20 μM SB203580 (lower middle panel) for 24 h prior to being probed with antiserum specific for p-ERK1/2 or total ERK1/2 (bottom panel). The decreases (n-fold) in ERK-MAPK phosphorylation are indicated in parentheses.