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. 2008 Jan 16;82(7):3342–3352. doi: 10.1128/JVI.02447-07

FIG. 6.

FIG. 6.

Assembly defects in W349A can be complemented by supplying NS3 protein in trans. (A) trans-complementation of W349A assembly defect by supplying NS3 or NS2B-3 protein alone. BHK-15 cells were electroporated with YF23-W349A transcripts, either alone or together with SIN-helper constructs expressing either NS2B-3 (+SIN-NS2B3) or NS3 (+SIN-NS3) proteins. (B) trans-complementation of W349A assembly defect by supplying enzymatically inactive NS3 protein. BHK-15 cells were electroporated with YF23-W349A transcripts, either alone or together with SIN helper constructs expressing either protease-inactive [+SIN-NS2B3(S138A)] or helicase-inactive [+SIN-NS2B3(R461Q)] NS2B-3 proteins. For both panels A and B, immunofluorescence assays were performed at 20 h (top panel) and 40 h (bottom panel) postelectroporation to detect E protein. The changes in the numbers of positive cells at 40 h compared to the numbers of positive cells at 20 h postelectroporation are reported as severalfold increases.