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. 2008 Jan 16;82(7):3271–3282. doi: 10.1128/JVI.01705-07

FIG. 1.

FIG. 1.

HCMV US6 inhibits cell surface expression of HLA-B2705. (A) Analysis of the effect of HCMV US6 on the expression of HLA-B2705 at the plasma membrane. HeLa-M cells were either transfected with a control vector or cotransfected with a control vector and the HLA-B2705 expression construct or cotransfected with HLA-B2705 and HCMV US6. To detect cell surface expression of HLA-B2705, the transfectants were stained with the ME1 MAb and a Cy5-conjugated secondary antibody. Antibody fluorescence was quantified by flow cytometry. The mean fluorescence value (MFV) for each of the transfected cell populations is indicated. (B) Analysis of the localization of HLA-B2705 in cells cotransfected with HCMV US6. HeLa-M cells were transfected with V5 epitope-tagged HLA-B2705 expression construct in combination with either a control vector or with HCMV US6-GFP. The transfected cells were stained with the anti-V5 epitope tag MAb SV5-Pk1, which detects the V5 epitope-tagged HLA-B2705 and an anti-mouse Texas red antibody. Antibody and GFP fluorescence was detected using a Zeiss Axioplan 2 microscope. The right panels are merged images of the red and green channels where yellow indicates colocalization; in these panels, the nuclei are also visualized with 4′,6′-diamidino-2-phenylindole (DAPI) (blue). Bars, 5 μm.