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Schematic representation of the HCMV US6 tetra-alanine mutants. The N-terminal signal sequence of US6 is indicated by the hatched box. The arrow indicates the predicted site for signal sequence cleavage. Residue 1 corresponds to the N terminus after signal sequence cleavage. The N-linked glycan (the circled G), predicted transmembrane domain (TMD), and C-terminal epitope tag incorporated into each construct (FLAG) are indicated.
