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. 1998 Jun 23;95(13):7568–7573. doi: 10.1073/pnas.95.13.7568

Figure 1.

Figure 1

Peptide-loaded HLA-A2/Ig specifically stains HTLV-1 Tax11–19 or HIV p17 Gag77–85-specific T cell clones. (A) Fluorescence-activated cell-sorting analysis was carried out using A6 cells (22), specific for the immunodominant HLA-A2-restricted Tax epitope Tax11–19. HTLV-1 Tax11–19-loaded HLA-A2/IgG stably bound on the cell surface and was detected using PE-labeled goat anti-mouse Ig (heavy line). Gag-A2/Ig was used as an irrelevant control (thin line) as well as no HLA-A2/Ig, unloaded HLA-A2/Ig, and M1-loaded HLA-A2/Ig (dotted and stripped lines overlapping). (B) T cells specific for the HLA-A2-restricted HIV p17 Gag77–85 epitope were stained to demonstrate the peptide specificity of peptide-loaded HLA-A2/Ig. Gag77–85-loaded HLA-A2/IgG was stably bound on the cell surface and detected using PE-labeled goat anti-mouse Ig (heavy line). HTLV-1 Tax11–19-loaded HLA-A2/Ig as well as no peptide loaded, M1-loaded HLA-A2/Ig, and no HLA-A2/Ig were used as an irrelevant control and stained virtually identical (overlapping thin, dotted, and stripped lines).