Figure 6.

Tax-specific CD8⁺ cells from HAM/TSP patients express intracellular IFN-γ and TNF-α. Using three-color flow cytometry, Tax-specific CD8⁺ cells were gated and analyzed for intracellular cytokine expression as described in Materials and Methods. Using mouse anti-human IFN-γ–PE, mouse anti-human TNF-α–PE, and an isotype-matched irrelevant mouse IgG1–PE control, 28% of the Tax-specific CD8⁺ cells from H1 (left panel) expressed intracellular IFN-γ and 29% expressed TNF-α; however, only 8% of the Tax-specific CD8⁺ cells from H6 (right panel) expressed intracellular IFN-γ and TNF-α.