FIG. 2.

Lactacystin acts directly on the cell proteasome and not on an HSV-specific function. CHO-nectin-1 cells were treated with lactacystin (A) or MG132 (B) for 30 min at 37°C. The medium was removed. Either the cells were subjected to four 5-min washes with culture medium, and then HSV-1 KOS-tk12 (MOI of 1) was added in medium without inhibitor (Wash), or else virus was added in the continued presence of agent (No Wash). The β-galactosidase activity was measured at 6 h p.i. (as in Fig. 1).