FIG. 7.

TRAIL is not required for apoptosis by Sendai virus. A. HT1080 cells were infected with Sendai virus as described in Materials and Methods. Cells were collected at the indicated times, and RNA was isolated. The amount of TRAIL message was determined by quantitative reverse transcription-PCR. B. HT1080 and P2.1 cells were pretreated with LY or vehicle control and infected with Sendai virus. Cells were harvested after 6 h, and RNA was isolated. TRAIL message was determined as for panel A. C. HT1080 cells were preincubated with LY294002 (50 μM) and subsequently infected or mock infected with Sendai virus. After infection, cells were washed and placed in complete medium containing LY294002. Anti-TRAIL blocking antibodies or DMSO vehicle control was added to the medium. Cells were fixed after 24 h.