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. 2008 Jan 23;82(7):3782–3790. doi: 10.1128/JVI.02076-07

FIG. 2.

FIG. 2.

Subcellular localization of picornavirus 2B proteins. (A to D, F, and H) BGM cells were cotransfected with EGFP-Golgi and the indicated 2B-myc constructs, fixed at 24 h posttransfection, and stained with anti-c-myc antiserum. (E, G, and I) BGM cells were transfected with the indicated 2B-myc constructs, fixed at 24 h posttransfection, and stained with anti-c-myc and anti-calreticulin antisera. Cells were analyzed by CLSM. The 2B-myc proteins of CBV3 (A), PV1 (B), and HRV14 (C) colocalized with the Golgi marker. HAV 2B did not colocalize with the Golgi marker (D) but was colocalized predominantly with the ER marker (E). FMDV 2B (F and G) and EMCV 2B (H and I) did not colocalize with the Golgi and ER markers. Bars = 10 μm.