FIG. 2.

(A) Wild-type cells were grown to exponential phase in YE5S, YE5S supplemented with 60 μM EDTA, or YE5S supplemented with 60 μM EDTA and 60 μM ZnSO₄. Cellular zinc contents were measured by atomic absorption spectrometry. Shown are the mean values from three experiments. Error bars indicate standard deviations. (B) Total RNA was prepared from cells grown as described for panel A and subjected to RNA blot hybridization using his3⁺ (control), zrt1⁺, and SPBC1348.06c probes. w.t., wild type.