FIG. 5.

(A) Wild-type (w.t.), zrt1-II1, and V7 strains were grown to exponential phase, subjected to fivefold serial dilutions, and spotted onto YE5S supplemented with the indicated concentrations of EDTA, ZnSO₄, FeCl₃, and CuSO₄. Plates were incubated at 30°C for 2 days. (B and C) The indicated strains were grown to exponential phase in YE5S or YE5S supplemented with the indicated concentrations of ZnSO₄. Cells were then harvested and processed for liquid β-galactosidase assays. Shown are the mean values (Miller units) from duplicate experiments.