FIG. 8.

(A) The indicated strains were grown to exponential phase, subjected to fivefold serial dilutions, and spotted onto EMM agar or EMM agar supplemented with ZnSO₄ (10 μM) and incubated at 30°C for the indicated times. (B) The indicated strains were grown for 25 h at 30°C in EMM or EMM supplemented with ZnSO₄ (50 μM). Shown are the mean values from three experiments. Error bars indicate standard deviations. Note that the wild-type (NT4) and fet4Δ (SW496) strains are ura4⁻, whereas the zrt1Δ (SW227) and fet4Δ zrt1Δ (SW500) strains are ura4⁺. wt, wild type.