TABLE 2.
Genes upregulated by zinc limitation
| Gene | Functiona | Relative mRNA level (level in zrt1-II1 cells/level in wild-type cells)
|
||
|---|---|---|---|---|
| Expt 1 | Expt 2 | Mean | ||
| SPBC1348.06c | Similar to SPBPB2B2.15 and SPAC977.05c | 165.91 | 194.24 | 180.1 |
| SPAC977.05c | Similar to SPBC1348.06C and SPBPB2B2.15 | 117.46 | 179.50 | 148.5 |
| adh4+ | Alcohol dehydrogenase | 42.52 | 56.47 | 49.5 |
| SPBPB2B2.15 | Similar to SPBC1348.06C and SPAC977.05c | 35.28 | 22.88 | 29.1 |
| zrt1+ (SPBC16D10.06) | ZIP zinc transporter | 9.72 | 14.04 | 11.9 |
| dak2+ | Dihydroxyacetone kinase | 5.14 | 10.26 | 7.7 |
| SPBP26C9.03c | Metal ion transporter similar to S. cerevisiae FET4 | 4.86 | 7.52 | 6.2 |
| SPCC18B5.02c | Cinnamoyl-coenzyme A reductase pseudogene | 3.27 | 4.65 | 4.0 |
| SPBC12C2.03c | Flavin adenine dinucleotide binding protein (predicted) | 3.30 | 4.40 | 3.9 |
| tlh1+ | RecQ type DNA helicase | 4.51 | 2.92 | 3.7 |
| SPAPB1A10.14 | F-box protein | 2.73 | 4.57 | 3.7 |
| SPAC23H3.15c | Sequence orphan | 5.64 | 1.53 | 3.6 |
| SPBC1652.01 | Predicted Sin3-interacting protein | 3.58 | 3.40 | 3.5 |
| SPAC977.04 | Pseudogene | 2.70 | 3.80 | 3.3 |
| SPAC212.09c | Pseudogene | 2.92 | 3.45 | 3.2 |
| SPAC750.08c | NAD-dependent malic enzyme | 2.88 | 3.28 | 3.1 |
| SPAPB18E9.04c | Glycoprotein (predicted) | 2.86 | 3.02 | 2.9 |
| mae2+ | Malate dehydrogenase | 2.71 | 3.15 | 2.9 |
| SPCC663.08c | Short-chain dehydrogenase (predicted) | 2.51 | 3.21 | 2.9 |
| SPAC57A10.06 | Sequence orphan | 2.54 | 3.16 | 2.9 |
| SPBPB7E8.01 | Glycoprotein (predicted) neutral zinc metallopeptidases | 3.12 | 2.54 | 2.8 |
| SPBC1348.05 | MFS family membrane transporter | 2.25 | 3.21 | 2.7 |
| SPAC22A12.06c | Serine hydrolase | 1.94 | 3.43 | 2.7 |
| obr1+ | Flavodoxin-like protein | 2.51 | 2.83 | 2.7 |
| SPCC663.06c | Short-chain dehydrogenase (predicted) | 2.48 | 2.81 | 2.6 |
| SPAC2H10.01 | Zn2-Cys6 transcription factor | 3.31 | 1.90 | 2.6 |
| SPAC977.07c | Glycoprotein | 1.50 | 3.64 | 2.6 |
| tpx1+ | Thioredoxin peroxidase | 2.48 | 2.60 | 2.6 |
| php2+ | CCAAT-binding factor complex subunit | 2.83 | 2.24 | 2.5 |
| SPBCPT2R1.07c | Malic pseudogene | 2.43 | 2.59 | 2.5 |
| SPAC13F5.03c | Glycerol dehydrogenase | 2.85 | 2.17 | 2.5 |
| SPCC794.01c | Glucose-6-phosphate 1-dehydrogenase | 2.27 | 2.74 | 2.5 |
| map2+ | Pheromone | 2.24 | 2.75 | 2.5 |
| rds1+ | Stress-induced protein | 3.42 | 1.56 | 2.5 |
| SPAC23C11.06c | Membrane protein | 3.13 | 1.77 | 2.5 |
| pex7+ | Peroxisomal signal receptor | 2.43 | 2.42 | 2.4 |
| gst2+ | Glutathione S-transferase | 2.36 | 2.45 | 2.4 |
| SPAC4F10.06 | Conserved fungal protein | 2.14 | 2.63 | 2.4 |
| srx1+ | Sulfiredoxin | 3.05 | 1.72 | 2.4 |
| SPAC4G8.03c | Pumilio family RNA binding protein | 2.31 | 2.39 | 2.4 |
| SPBC8E4.02c | Sequence orphan | 2.20 | 2.49 | 2.3 |
| SPAC22A12.17c | Short-chain dehydrogenase (predicted) | 2.36 | 2.31 | 2.3 |
| SPAC16.05c | Transcription factor similar to those of S. cerevisiae SFP1 | 2.29 | 2.32 | 2.3 |
| SPAC1786.02 | Phospholipase (putative) | 2.12 | 2.42 | 2.3 |
| SPBC1773.13 | Aromatic aminotransferase (predicted) | 2.34 | 2.13 | 2.2 |
| SPCC1235.01 | Glycoprotein (predicted) | 2.05 | 2.41 | 2.2 |
| SPCC569.05c | Spermidine family transporter (predicted) | 2.11 | 2.28 | 2.2 |
| SPAC5H10.04 | NADPH dehydrogenase | 2.03 | 2.31 | 2.2 |
| SPAP27G11.08c | Meiotic expression upregulated | 1.72 | 2.62 | 2.2 |
| SPBC16E9.16c | Sequence orphan | 2.44 | 1.88 | 2.2 |
| SPCC1223.13 | Transcription factor (predicted) | 2.27 | 2.05 | 2.2 |
| meu26+ | Conserved fungal protein | 2.00 | 2.29 | 2.1 |
| SPBPB2B2.19c | Membrane protein | 2.01 | 2.28 | 2.1 |
| SPAC19B12.10 | AMSH protein homolog | 2.11 | 2.16 | 2.1 |
| pof1+ | F-box protein | 2.10 | 2.16 | 2.1 |
| caf5+ | Spermine transporter family | 1.89 | 2.34 | 2.1 |
| SPBC409.08 | Spermine transporter family | 1.89 | 2.22 | 2.1 |
| tht1+ | Nuclear membrane protein involved in karyogamy | 2.05 | 2.05 | 2.1 |
| gst1+ | Glutathione S-transferase | 2.15 | 1.93 | 2.0 |
| atf31+ | bZIP transcription factor | 2.01 | 2.02 | 2.0 |
| spd1+ | Ribonucleotide reductase inhibitor | 1.96 | 2.06 | 2.0 |
| SPCC1020.03 | Mitochondrial ion transporter | 1.59 | 2.37 | 2.0 |
| SPBC1348.02 | S. pombe-specific protein | 1.88 | 2.08 | 2.0 |
a
The descriptions of the protein functions are based on information for S. pombe in GeneDB (http://www.genedb.org/genedb/pombe/index.jsp).