FIG. 2.

GXM shed from cells grown under capsule-inducing conditions displays electrophoretic mobility that is lower than that for cells grown in rich medium. Cultures of JEC21 cells were grown overnight and then washed and diluted to 10⁶ cells/ml for continuous growth as indicated, followed by analysis of GXM as described for Fig. 1 (lanes rotated 90 degrees counterclockwise). Top lane, cells grown in YPD for 75 h, to a final concentration of 4 × 10⁸ cells/ml; bottom lane, cells grown in DMEM (Sigma catalog no. D5796) in the presence of 5% CO₂ at 30°C for 14 h, to a final concentration of 1.6 × 10⁶ cells/ml. Note that the growth conditions for the YPD sample, 75 h of continuous growth, correspond to those that yielded the slowest-migrating material in Fig. 1.