FIG. 3.

Capsule size correlates with the electromobility of shed GXM. Capsule-induced JEC21 cells were washed and resuspended in YPD, and samples were taken at 4, 8, and 24 h after the medium change. (A) Two representative micrographs of cells at each time point, imaged as described in Table 1; scale bar, 10 μm. (B) Capsule thickness of 50 cells was measured as in Table 1, with frequency plotted as a histogram (bin width equals 1 μm with the bin maxima indicated on the abscissa, in decreasing order). (C) Electromobility of shed GXM, analyzed as described for Fig. 1. Ind, original induced sample; 4, 8, and 24 indicate hours after medium change.