TABLE 1.
Hour | Continuous culture
|
Diluted culture
|
||
---|---|---|---|---|
Cell densitya | Capsule thicknessb | Cell density | Capsule thickness | |
11 | 9.1 × 107 | 0.80 ± 0.03 | 9.1 × 107 | 0.80 ± 0.03 |
24 | 1.5 × 108 | 1.35 ± 0.06 | 8.6 × 107 | 0.90 ± 0.02 |
35 | 1.8 × 108 | 1.37 ± 0.07 | 6.4 × 107 | 0.82 ± 0.03 |
48 | 2.1 × 108 | 1.60 ± 0.19 | 1.0 × 108 | 1.02 ± 0.05 |
75 | 4.3 × 108 | 1.54 ± 0.08 | 1.7 × 108 | 0.93 ± 0.02 |
Cell density at the indicated time of growth (cells/ml), determined by counting cells on a hemocytometer.
Capsule thickness of India-ink-stained cells, measured using an Axioskop 2 fluorescence microscope (Carl Zeiss). The averages ± standard errors of the means (μm) of 50 cells for each time point are tabulated.