FIG. 3.

Effects of protease inhibitors on IL-8 production from T84 cells induced by B. ratti WR1. T84 cells were coincubated for 24 h with WR1 lysate pretreated with one of several protease inhibitors. Cysteine protease inhibitors, iodoacetamide and E-64, most significantly reduced WR1-induced IL-8 production from T84 cells (*, P = 0.002, and **, P = 0.007, versus WR1 lysate). Considerable reduction in IL-8 production was seen with the aspartic inhibitor pepstatin A (##, P = 0.04 versus WR1 lysate). Minimal inhibition was observed with serine and metalloprotease inhibitors, PMSF and EDTA, respectively. The values are means ± standard deviations (n = 3). #, P < 0.02 versus the control.