Fig. 5.

Human tracheal costaining of SC18 and NY18 HA with Jacalin. The apical side of the tracheal epithelia is indicated by white arrows. The distinct apical binding patterns of SC18 and NY18 (in Fig. 4) are further elaborated by costaining the HAs (red) with Jacalin (green), a marker for goblet cells. The images were taken at ×25 magnification, and representative regions of the apical side are enlarged for clarity. The significant costain of SC18 HA with Jacalin (yellow) indicates that SC18 HA predominantly binds to goblet cells on the apical side of the epithelia. On the other hand, the binding pattern of NY18 HA has minimal overlap with that of Jacalin, indicating that it does not bind to goblet cells on the apical side of tracheal tissue.