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AFM experiment. (A) A typical experimental AFM force–extension trace for the desorption of a single C16 spider silk peptide chain from a hydrophobic diamond surface in 20 mM NaCl, exhibiting a force plateau in the extension range 125 nm < z < 234 nm. (B). Scheme of the setup. (C and D) Distribution for 200 traces with the identical peptide chain for the force plateau length (C) and the plateau height (D), giving a mean desorption force of F = 58 ± 8 pN.
