Fig. 2.

Expression profiles of five members of the miR-17-92 cluster over seven time points with and without hormonal induction. (A) Box plots showing the data distribution of the miR-17-92 cluster over time. The horizontal lines in the boxes indicate the median values; the lower and upper edges of the boxes indicate the 25th and 75th percentiles of the datasets, respectively; the whiskers indicate the minimum and maximum values (or 1.5 times the interquartile range). The red- and yellow-colored boxes represent cells grown in differentiation and growth medium, respectively. (B) Cluster analysis for all five members of miR-17-92 cluster available on Invitrogen miRNA chips. The red color shows relatively abundant expression of the same miRNA compared with other time points, whereas the blue color indicates a low expression relative to others. Cluster analyses were performed with GeneSpring GX 7.3.1 by using the default settings. (C) The relative expression levels of miR-17-5p and miR-18 at days 1, 2, and 6 after differentiation, as detected by QRT-PCR. The red line represents miR-17-5p, and the blue line denotes miR-18. The data shown are means and SDs of three replicates.