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. 2008 Feb 19;105(8):2889–2894. doi: 10.1073/pnas.0800178105

Fig. 4.

Fig. 4.

Rb2/p130 is a direct target of miR-17-92. (A) Rb2/p130 mRNA in the cells transfected with miR-17-92 was lower than that in the control cells at different time points of hormonal induction. (B) Rb2/p130 protein in the cells transfected with miR-17-92 was markedly reduced compared with the cells transfected with empty MSCV vector. (C) Luciferase activities were decreased in MES13 cells in a dose-dependent fashion by transfection of different concentration of synthetic miR-17-5p. There was no difference in luciferase activities between the cells harboring the pGL3-Rb2/p130 construct and the cells harboring the pGL3 control construct before cotransfection. The luciferase activities were decreased with the increase of synthetic miR-17-92 concentrations. ***, P < 0.001 between 0 and 60 nM; *, P < 0.05 between 60 and 80 nM. There was no significant difference between 80 and 100 nM, which could be attributable to the saturation of synthetic miR-17-5p at 80 nM.