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Genetic dependence of PolIV-dependent loci. siRNA Northern blots of mixed floral tissue confirm that all tested PolIV-dependent loci lose siRNAs in nrpd1a. They also require NRPD2, RDR2, and DCL3 for siRNA accumulation but show various degrees of dependence on NRPD1b. The size markers are estimates based on 20- and 30-nt RNA oligos.
