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Dual-level targeting allows specific 5′-UTR-E1A mRNA translation during CRAd infection. Western blot analysis was performed using lysates from normal cells expressing relatively low levels of eIF4E (A) and breast cancer cell lines expressing relatively high levels of eIF4E (B) after infection with after infection with Ad-CXCR4-E1A, Ad-CXCR4-UTR-E1A, Ad-CXCR4-GL3 (negative control) or Ad-wt-dE3 (positive control).
